Chromosomal DNA extraction and you may optical map research order

Chromosomal DNA extraction and you may optical map research order Botrytis cinerea protoplasts was in fact inserted within the 0.5% reduced melting temperatures agarose gel inserts (Schwartz and Cantor, 1984 ), right after which lysed playing with 0.5 m ethylenediaminetetraacetic acid (EDTA), 1% lauroyl sarcosine, 2 mg/mL proteinase K, 1 meters NaCl, pH 9.5, for…

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